RNA
Part:BBa_K2510055
Designed by: Joseph Ryan Group: iGEM17_Paris_Bettencourt (2017-10-27)
49xCAG Repeats
49xCAG Repeats
Usage and Biology
The 49xCAG sequence was built using two oligonucleotides: 10xCAG and 10xCTG. Through testing a variety of oligonucleotide concentrations and testing a different of PCR conditions, a specific protocol was built to synthesise various lengths of DNA containing CAG repeats.
The 49xCAG repeats were used in FA-FB System where the FA component is expressed an MS2 aptamer, which is a specific bacteriophage binding site that connects an MS2 binding domain. By expressing the CAG repeat sequence with an MS2 binding domain, specific binding can occur on the RNA aggregation. Thus, this creates a localization of FA in the cell.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |